All column hardware is 316 stainless steel. The end fittings are compatible with fittings from Valco, Upchurch, Parker, Swagelok and others. However, all connecting fittings should be assembled in your new column to ensure a proper fit. Removing or tampering of the column end fittings invalidates the warranty.
Connecting your Column :-
The column is shipped containing the mobile phase indicated on the QC test, overleaf. Ensure that your mobile phase is compatible with this. If not, you must flush with an intermediate solvent that is compatible with the storage solvent and your mobile phase. Be especially careful if you are using a buffer because most columns are shipped with more than 50% organic solvent, which could cause precipitation of the buffer and plugging of the column. Failure to follow this washing protocol will invalidate the column warranty.
Flow Direction : –
The direction of flow is indicated on the column label. Begin by connecting the inlet end of the column to the injector or Autosampler and gradually increase the flow rate to its optimum value.
Column Lifetime :-
PerkinElmer columns are packed with silica particles. The normal limitations are from pH 2.5 to pH 7.5. Extended use at either extreme of pH may shorten the lifetime of column from the optimum.
Column Clean-Up :-
Column lifetime can be extended considerable with routine column washes and proper storage. Columns should be flushed when not in use to remove buffers, acids or bases. The ideal flushing solvent is identical in composition to your mobile phase, minus the acid, base or buffer.